Sad news today – Roger Tsien passed away one week ago.
Can anyone imagine biology today without GFP? And though he is best known for that – he did share the Nobel prize for GFP, after all – Roger Tsien did much more. One of my favorite stories about Roger (and he was a character; you couldn’t be at UCSD without one or two Roger stories) came from a talk he gave while I was there. He was asked to step in at a somewhat late moment to give a series of three one-hour lectures on his life’s work. It was one of the best talks I have attended, clear and insightful and packed with interesting anecdotes.
He begun describing his struggles as a graduate student, how he had a hard time doing electrophysiology. His solution? He created BAPTA, the calcium chelator that is the basis for neural imaging. Yeah: electrophysiology too hard? Just create the basis for calcium imaging. Much easier. (And he was quite honest; it was much easier for him.)
You should of course read that original BAPTA paper (this is under-appreciated!). Then of course there is his GFP paper, establishing a point mutation with much improved characteristics. Here is his Nobel lecture. I don’t think it’s an understatement to say that these are just the tip of the iceberg.